G4 Prediction Server

G-quadruplexes (G4) are unique secondary structures formed in guanine-rich regions of DNA or RNA. Comprising stacks of guanine tetrads held together by Hoogsteen hydrogen bonds, these structures play crucial roles in various biological processes, including maintaining genomic integrity, regulating transcription, and protecting telomeres. GPS is an intuitive, machine-learning-based tool designed for comprehensive G4 analysis, with three main functionalities. First, it runs RNAfold to predict secondary structures; second, it calculates G4-specific features based on RNAfold results; and third, it predicts G4 motifs and evaluates their folding status. The tool accepts input in three formats: FASTA, a CSV file with sequences in a dedicated column, and individual sequences, allowing users flexibility in data submission for efficient G4 analysis. With its powerful capabilities and user-friendly interface, GPS redefine G4 analysis, making advanced insights accessible to researchers with ease and precision.